MMP2 Antibody

SKU: orb750021

Description

It recognizes a protein of 72 kDa, which is identified as MMP2. The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, Fibronectin, Laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP2 (also designated type IV collagenase) cleaves collagen types IV,V, VII and X and gelatin type I. Activation of MMP2 secretion requires the Ras signaling pathway.

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Cancer,Product Categories/Products/Antibodies/Primary Antibodies,Epigenetics

Images & Validation

• Tested Applications: WB
• Dilution Range: Western blot: 0.5-1ug/ml
• Reactivity: Human
• Application Notes:
  The stated application concentrations are suggested starting amounts. Titration of the MMP2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: MMP2/1501
• Immunogen: Amino acids 195-319 were used as the immunogen for the MMP2 antibody.
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store the MMP2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Hazard Information: This MMP2 antibody is available for research use only.
• Disclaimer: For research use only

Western blot testing of 1) a partial recombinant protein and 2) human U87 cell lysate with MMP2 antibody (clone MMP2/1501). Expected molecular weight: ~72 kDa (pro form), ~63 kDa (cleaved form).

SDS-PAGE Analysis of Purified, BSA-Free MMP2 Antibody (clone MMP2/1501). Confirmation of Integrity and Purity of the Antibody.

Quick Database Links

UniProt

P08253