MMP9 Antibody: APC

SKU: orb149758

Description

Mouse monoclonal to MMP9 (APC). MMP9, otherwise known as matrix metalloproteinase 9, is involved in the breakdown of extracellular matrix in normal physiological processes such as embryonic development, reproduction and tissue remodeling, as well as in disease processes like arthritis and metastasis. Among the family members, MMP-2, MMP-3, MMP-7 and MMP-9 have been characterized as important factors for normal tissue remodeling during embryonic development, wound healing, tumor invasion, angiogenesis, carcinogenesis and apoptosis (2-4). MMP activity correlates with cancer development. One mechanism of MMP regulation is transcriptional. Once synthesized, MMP exists as a latent proenzyme. Maximum MMP activity requires proteolytic cleavage to generate active MMPs by releasing the inhibitory propeptide domain from the full length protein..

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Cardiovascular Disease,Epigenetics,Cardiovascular System | Angiogenesis | Cardiovascular Growth Factors | Adhesion / Extracellular Matrix | Matrix Metalloproteinases | Cell Signaling | Extracellular Matrix (ECM) | Enzymes | Cell Signaling | Extracellular Matrix (ECM) | Enzymes | Cancer | Tumor Biomarkers | Tumor Biomarker Enzymes | Cancer | Invasion/microenvironment

Images & Validation

• Tested Applications: ICC, IF, IHC
• Dilution Range: WB (1:1000), ICC/IF (1:100)
• Reactivity: Human, Mouse, Rat
• Application Notes:
  1 µg/ml of SMC-396 was sufficient for detection of MMP9 in 20 µg of COS-1 cells (lysate) transfected with human MMP9 by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone No.: L51/82 (Formerly sold as S51-82)
• Immunogen: Fusion protein amino acids 1-708 (full length) of rat MMP9
• Target: MMP9
• Molecular Weight: 82kDa
• Conjugation: APC

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 95.64mM Phosphate, 2.48mM MES and 2mM EDTA
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

MMP-9 antibody, CLG4B antibody, 82kDa matrix metalloproteinase-9 antibody, collagenease type 4 beta antibody, GELB antibody, Macrophage gelatinase antibody, MANDP2 antibody, Type V collagenase antibody, 92 kDa gelatinase antibody, 92 kDa type IV collagenase antibody, Gelatinase B antibody, MMP9 antibody, Matrix metalloproteinase 9 antibody

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-MMP9 Monoclonal Antibody, Clone L51/82. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-MMP9 Monoclonal Antibody at 1:50 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) MMP9 Antibody (D) Composite.

Western Blot analysis of Rat Brain showing detection of ~92 kDa and ~82 kDa (pro and active) MMP9 protein using Mouse Anti-MMP9 Monoclonal Antibody, Clone L51/82. Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain. Load: 15 μg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-MMP9 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~92 kDa and ~82 kDa (pro and active).

Quick Database Links

Gene Symbol

MMP9

UniProt

P50282

NCBI Gene ID

81687

RefSeq (Protein):NP_112317.1