Moesin Antibody

SKU: orb2640131

Description

Moesin, a member of the talin-4.1 superfamily, is a linking protein of the sub-membranous actin cytoskeleton.

Images & Validation

• Tested Applications: FACS, IF, IHC-P, WB
• Dilution Range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 2-4ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Reactivity: Human, Rat
• Application Notes:
  Titering of the Moesin antibody may be required for optimal performance.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: MSN/491
• Immunogen: Recombinant full-length human protein was used as the immunogen for the Moesin antibody.
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Hazard Information: This Moesin antibody is available for research use only.
• Disclaimer: For research use only

IHC testing of FFPE human melanoma with Moesin antibody (clone MSN/491). Required HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min.

IHC testing of FFPE human placenta with Moesin antibody (clone MSN/491). Required HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min.

IHC testing of FFPE rat colon with Moesin antibody (clone MSN/491). Required HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min.

IHC testing of FFPE rat lung with Moesin antibody (clone MSN/491). Required HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min.

Western blot testing of human HT29 cell lysate with Moesin antibody (clone MSN/491). Predicted molecular weight ~68 kDa but routinely observed at 68-78 kDa.

SDS-PAGE Analysis of Purified, BSA-Free Moesin Antibody (clone MSN/491). Confirmation of Integrity and Purity of the Antibody.

Western blot testing of human PC3 cell lysate with Moesin antibody (clone MSN/491). Predicted molecular weight ~68 kDa but routinely observed at 68-78 kDa.

Flow cytometry testing of permeabilized human K562 cells with Moesin antibody (clone MSN/491); Red = isotype control, Blue = Moesin antibody.

Immunofluorescent staining of PFA-fixed human HeLa cells with Moesin antibody (clone MSN/491, green) and Reddot nuclear stain (red).

Quick Database Links

UniProt

P26038