Mouse anti-CD20 Monoclonal Antibody, Purified [L26]

SKU: orb1520131

This product can be custom made

Description

CD20 Antibody

Research Area

Product Categories/Products/Antibodies,Primary Antibodies

Images & Validation

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Item 1 of 2

Tested Applications	FC, IF, IHC, WB
Dilution Range	WB - 1:1000; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.; IHC-IF - 1:100 - 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Reactivity	Human
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Immunogen	Human tonsil B cells
Target	CD20
Conjugation	Unconjugated

Customer Validated Data

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The data below is submitted by researchers worldwide. We provide this transparency to help you decide if this product works for your specific application or species, even if we haven't tested it ourselves.

Success by Application

Reactivity Distribution

Latest Experiments

1 Results

Dilution

Sample

Summary

Human

Tissue microarrays (TMA) constructed from FFPE

Sample preparation involved deparaffinizing TMA sections...

Storage & Handling

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Storage	2 - 8°C
Form/Appearance	Whole IgG
Buffer/Preservatives	Phosphate Buffered Saline (PBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
Concentration	300 µg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Membrane-spanning 4-domains subfamily A member 1; S7; membrane-spanning 4-domains, subfamily A, member 1; leukocyte surface antigen Leu-16; LEU-16; CVID5; CD20 receptor; CD20; CD antigen CD20; Bp35; B-lymphocyte surface antigen B1; B1; B-lymphocyte cell-surface antigen B1; B-lymphocyte antigen CD20; CD20 antigen; MS4A2

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Mouse anti-CD20 Monoclonal Antibody, Purified [L26]

Detection of human CD20 (shaded) in Raji cells by flow cytometry. Antibody: Mouse anti-CD20 monoclonal antibody [L26] (orb1520131) or isotype control (unshaded). Secondary: DyLight® 488-conjugated goat anti-mouse IgG.

Detection of human CD3E (yellow) , CD8 alpha (red) , and CD20 (green) in FFPE tonsil by IHC-IF. Rabbit anti-CD3E recombinant monoclonal [BL-298-5D12], rabbit anti-CD8 alpha recombinant monoclonal, mouse anti-CD20 monoclonal [L26]. Secondary: HRP-conjugated goat anti-rabbit IgG and HRP-conjugated goat anti-mouse IgG. Substrate: Opal™ 520, 620, and 690. Counterstain: DAPI (blue).

Quick Database Links

Gene Symbol

CD20

UniProt

P11836

RefSeq (Protein):NP_690605.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_690605.1

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Protocol Information

Western Blot (IB, immunoblot)

View Protocol

IHC

Immunohistochemistry

Flow Cytometry

Immunofluorescence