Mouse anti Human CD4, conjugated with Biotin

SKU: orb1570894

Description

Mouse anti Human CD4, conjugated with Biotin

Research Area

,Flow Cytometry

Images & Validation

• Tested Applications: FC
• Reactivity: Human
• Application Notes:
  PBMC: Add10 µl of MAB/106 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2o to 8oC. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20oC. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. ALLOPHYCOCYANIN : (APC) conjugates are analyzed in multi-color flow cytometry with instruments equipped with a second laser and proper filters. Laser excitation is at 633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633 nm) range for a Dye laser. Peak fluorescence emission is at 660 nm. RPE-Cy-5 +: Use 5 µl of solution, rather than 10 µl as above. Excites at 488nm and emits at 670nm. Store protected from light.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 7E14
• Immunogen: Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human perherial blood T lymphocytes.
• Conjugation: Biotin

Storage & Handling

• Storage: Product should be stored at 4-8°C. DO NOT FREEZE
• Buffer/Preservatives: Provided as solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein
• Concentration: Titered for flow cytometry
• Expiration Date: 12 months from date of receipt.
• Hazard Information: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
• Disclaimer: For research use only

Alternative Names

T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3

Quick Database Links

UniProt

P01730