Mouse anti Human IgA2 (subclass specific), conjugated with FITC

SKU: orb22234

Description

Fluorescein isothiocyanate-conjugated purified monoclonal mouse antibody to human IgA2, subclass specific. To identify the presence of IgA2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as immunofluorescence staining of cytoplasmic IgA2, and immunoblotting using a peroxidase labelled monoclonal antibody against FITC. The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:20 to 1:100; in Western blotting from 1:200 upwards. Working dilutions may vary widely, strongly depending on the test conditions. These data should be interpreted as general recommendations only.

Images & Validation

• Reactivity: Human

Key Properties

• Antibody Type: Secondary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: NI 512 (A89-038)
• Conjugation: FITC

Storage & Handling

• Storage: The lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted solutions should be stored at +4°C, not refrozen, and preferably used the same day.
• Buffer/Preservatives: Purified monoclonal mouse IgG1 kappa conjugated with FITC, lyophilized from a solution in phosphate buffered saline (pH7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added.
• Expiration Date: 12 months from date of receipt.
• Hazard Information: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Biorbyt accepts no liability for any inaccuracies or omissions in this information.
• Disclaimer: For research use only

Oka, Kazumasa et al. IgA1 dominant subclass of latent IgA mesangial deposition in donated kidney Int J Nephrol Renovasc Dis, 9, 313-317 (2016)

PubMed: 27942230