Mouse Complement C5 Protein, His Tag

SKU: orb653662

Description

Mouse Complement C5, His Tag is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Glu 1680 (Accession # P06684-1).

Research Area

Immunology & Inflammation

Images & Validation

• Application Notes:
  This protein carries a polyhistidine tag at the C-terminus. The mature form of Complement C5 is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain. Each α and β chain has a calculated MW of 73.5 kDa (β chain) and 114.6 kDa (α chain). The protein migrates as 66 kDa (β chain) and 100 kDa (α chain) under reducing (R) condition (SDS-PAGE) due to glycosylation.

Key Properties

• Expression System: HEK293
• Biological Origin: Mouse
• Biological Activity: Immobilized Mouse Complement C5, His Tag at 2 µg/mL (100 µL/well) can bind Monoclonal Anti-Human C5a Human Antibody, Human IgG1 with a linear range of 0.1-13 ng/mL (QC tested).
• Target: Complement C5
• Reactivity: Mouse
• Tag: C-10×His
• Expression Region: Gln 19 - Glu 1680
• Endotoxins: 1.0 EU per μg
• Conjugation: Unconjugated
• MW: 73.5 kDa (β chain) & 114.6 kDa (α chain)
• Purity: 95%
• Protein Sequence: Gln 19 - Glu 1680

Storage & Handling

• Storage: -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
• Form/Appearance: Powder
• Buffer/Preservatives: PBS, pH 7.4
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Immobilized Mouse Complement C5, His Tag (Cat. No. orb653662) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-Human C5a Human Antibody, Human IgG1 with a linear range of 0.1-13 ng/mL (QC tested).

Quick Database Links

Gene Symbol

Complement C5

UniProt

P06684