(Mouse) Epcam Antibody (C-term)

SKU: orb306604

Description

Rabbit polyclonal antibody to Epcam

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Product Categories/Products/Antibodies/Primary Antibodies,Epigenetics,Crown Antibodies;Primary Antibodies

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB: 1:2000
• Reactivity: Human, Mouse, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB52384
• Target: Epcam
• Molecular Weight: 35019
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti Epithelial cell adhesion molecule antibody, anti Ep-CAM antibody, anti Epithelial glycoprotein 314 antibody, anti EGP314 antibody, anti mEGP314 antibody, anti Protein 289A antibody, anti Tumor-associated calcium signal transducer 1 antibody, anti CD326 antibody, anti Epcam antibody, anti Tacstd1 antibody

All lanes: Anti-Epcam Antibody (C-term) at 1:2000 dilution. Lane 1: mouse lung lysates. Lane 2: mouse kidney lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 35 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-Epcam Antibody (C-term) at 1:2000 dilution. Lane 1: mouse kidney lysates. Lane 2: mouse colon lysates. Lane 3: MCF-7 whole cell lysates. Lane 4: rat colon lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 35 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Staining (Mouse) Epcam in mouse colon sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Quick Database Links

Gene Symbol

Epcam

UniProt

Q99JW5