Mouse GCSF protein

SKU: orb80032

Description

Granulocyte MCSF Mouse Recombinant produced in E.coli is a single, non-glycosylated, polypeptide chain containing 125 amino acids and having a molecular mass of 14.2 kDa.GMCSF Mouse is purified by proprietary chromatographic techniques.

Images & Validation

• Tested Applications: FA, HPLC, SDS-PAGE
• Application Notes:
  < strong>Experiment Notes: GM-CSF quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.765 as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using calibrated solution of GM-CSF as Reference Standard.

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Key Properties

• Biological Origin: E.coli
• Biological Activity: The ED50 as determined by the dose-dependant stimulation of the proliferation of murine FDC-P1 cell line is 0.2 ng/ml, corresponding to Specific Activity of 5,000,000 IU/mg.
• Target: mGM CSF
• Conjugation: Unconjugated
• Purity: > 98.0% as determined by RP-HPLC and analysis by SDS-PAGE
• Protein Sequence: MAPTRSPITV TRPWKHVEAI KEALNLLDDM PVTLNEEVEV VSNEFSFKKL TCVQTRLKIF EQGLRGNFTK LKGALNMTAS YYQTYCPPTP ETDCETQVTT YADFIDSLKT FLTDIPFECK KPVQK

Storage & Handling

• Storage: Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
• Buffer/Preservatives: GM-CSF Mouse was lyophilized with no additives.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CSF-2 protein, MGI-1GM protein, GM-CSF protein, Pluripoietin-alpha protein, Molgramostin protein, Sargramostim protein

Quick Database Links

Gene Symbol

mGM CSF