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Mouse GRO/KC protein

SKU: orb80129

Description

KC Mouse Recombinant also known as N51 and GRO1 produced in E.coli is a single,non-glycosylated, polypeptide chain containing 77 amino acids and having a molecular mass of approximately kDa.The GRO-1 is purified by proprietary chromatographic techniques.

Images & Validation

Tested ApplicationsFA, HPLC, SDS-PAGE
Application Notes
< strong>Experiment Notes: Protein quantitation was carried out by two independent methods 1. UV spectroscopy at 280 nm using the absorbency value of 0.03 as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using standard solution of KC as Reference Standard.

Key Properties

Biological OriginE.coli
Biological ActivityThe biological activity was determined by measuring the dose dependent mobilization of intracellular calcium (calcium flux) with human neutrophils. Significant calcium mobilization is observed with 50ng/mL of recombinant mouse KC (Specific Activity: 2 104 units/mg).
TargetmGRO1/KC
ConjugationUnconjugated
Purity> 95.0% as determined by RP-HPLC and analysis by SDS-PAGE
Protein SequenceRLATGAPIAN ELRCQ CLQT MAGIHL KNI QSLKVLP SG PHCTQTEV I ATLKNGREA CLDPEAPLVQ KIVQKMLKG V PK.

Storage & Handling

StorageStore at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
Buffer/PreservativesThe protein was lyophilized with no additives.
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Growth-regulated alpha protein protein, CXCL1 protein, Platelet-derived growth factor-inducible protein KC protein, Secretory protein N51 protein, KC protein, Fsp protein, N51 protein, gro protein, Gro1 protein, Mgsa protein, Scyb1 protein, chemokine (C-X-C motif) ligand 1 protein
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Protocol Information

Protein Handling and Storage Guide
Protein Handling Guide
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SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
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HPLC
High-Performance Liquid Chromatography
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Available Sizes

Select a size below

5 μg
$ 170.00
100 μg
$ 960.00
500 μg
$ 1,910.00
DispatchUsually dispatched within 2-3 weeks
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