Mouse IFN-alpha 1 Protein, His Tag

SKU: orb735042

Description

Mouse IFN-alpha 1, His Tag is expressed from human 293 cells (HEK293). It contains AA Cys 24 - Lys 189 (Accession # P01572-1).

Research Area

Cytokines

Images & Validation

• Application Notes:
  This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 21.0 kDa. The protein migrates as 24-27 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Key Properties

• Expression System: HEK293
• Biological Origin: Mouse
• Biological Activity: Loaded Mouse IFN-alpha 1, His Tag on HIS1K Biosensor, can bind Human IFNAR1, Fc Tag with an affinity constant of 0.17 µM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
• Target: IFN-alpha 1
• Reactivity: Mouse
• Tag: C-10×His
• Expression Region: Cys 24 - Lys 189
• Endotoxins: 1.0 EU per μg
• Conjugation: Unconjugated
• MW: 21.0 kDa
• Purity: 90%
• Protein Sequence: NP_034632.2

Storage & Handling

• Storage: -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
• Form/Appearance: Powder
• Buffer/Preservatives: PBS, pH 7.4
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Loaded Mouse IFN-alpha 1, His Tag (Cat. No. orb735042) on HIS1K Biosensor, can bind Human IFNAR1, Fc Tag (Cat. No. orb257558) with an affinity constant of 0.17 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Quick Database Links

Gene Symbol

IFN-alpha 1

UniProt

P01572

RefSeq (Protein):NP_034632.2