Mouse IgG (H&L) Antibody Peroxidase Conjugated

SKU: orb347619

Description

Mouse IgG (H&L) antibody (Peroxidase)

Research Area

Product Categories/Products/Antibodies,Immunogen Type: Native Protein

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:100,000 - 1:200,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:5,000
• Reactivity: Mouse
• Application Notes:
  Anti-Mouse IgG Peroxidase Conjugated Antibody has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Sheep
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-Mouse IgG [H&L] was produced by repeated immunization with Mouse IgG whole molecule in sheep.
• Purity: Sheep Anti-Mouse IgG[H&L] Peroxidase Conjugated Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Sheep Serum, Mouse IgG and Mouse Serum.
• Conjugation: HRP

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Concentration: 2.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

sheep anti-Mouse IgG heavy and light chain peroxidase conjugated Antibody, sheep anti-Mouse IgG HRP secondary Antibody, sh anti-ms IgG HRP antibody