Mouse IL-3 Protein, His Tag

SKU: orb1101195

Description

Mouse IL-3 Protein, His Tag is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Cys 166 (Accession # P01586-1).

Research Area

Immunology & Inflammation

Images & Validation

• Application Notes:
  This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 17.6 kDa. The protein migrates as 25-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Key Properties

• Expression System: HEK293
• Biological Origin: Mouse
• Biological Activity: Immobilized Mouse IL-3 R alpha, His Tag on CM5 Chip can bind Mouse IL-3 Protein, His Tag with an affinity constant of 0.929 µM as determined in a SPR assay (Biacore T200) (QC tested).
• Target: IL-3
• Reactivity: Mouse
• Tag: C-10×His
• Expression Region: Ala 27 - Cys 166
• Endotoxins: 1.0 EU per μg
• Conjugation: Unconjugated
• MW: 17.6 kDa
• Purity: 90%
• Protein Sequence: NP_034686.2

Storage & Handling

• Storage: -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
• Form/Appearance: Powder
• Buffer/Preservatives: PBS, pH 7.4
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Immobilized Mouse IL-3 R alpha, His Tag on CM5 Chip can bind Mouse IL-3 Protein, His Tag (Cat. No. orb1101195) with an affinity constant of 0.929 μM as determined in a SPR assay (Biacore T200) (QC tested).

Quick Database Links

Gene Symbol

IL-3

UniProt

P01586

RefSeq (Protein):NP_034686.2