Mouse IL-6 Protein, His Tag

SKU: orb750344

Description

Mouse IL-6, His Tag is expressed from human 293 cells (HEK293). It contains AA Phe 25 - Thr 211 (Accession # P08505-1).

Research Area

Immunology & Inflammation

Images & Validation

• Application Notes:
  This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 23.6 kDa. The protein migrates as 25-35 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Key Properties

• Expression System: HEK293
• Biological Origin: Mouse
• Biological Activity: Immobilized Mouse IL-6, His Tag at 5 µg/mL (100 µL/well) can bind Biotinylated Mouse IL-6 R alpha, His, with a linear range of 20-156 ng/mL (QC tested).
• Target: IL-6
• Reactivity: Mouse
• Tag: N-10×His
• Expression Region: Phe 25 - Thr 211
• Endotoxins: 1.0 EU per μg
• Conjugation: Unconjugated
• MW: 23.6 kDa
• Purity: 90%
• Protein Sequence: NP_112445.1

Storage & Handling

• Storage: -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
• Form/Appearance: Powder
• Buffer/Preservatives: PBS, pH 7.4
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Immobilized Mouse IL-6, His Tag (Cat. No. orb750344) at 5 μg/mL (100 μL/well) can bind Biotinylated Mouse IL-6 R alpha, His, Avitag with a linear range of 20-156 ng/mL (QC tested).

Quick Database Links

Gene Symbol

IL-6

UniProt

P08505

RefSeq (Protein):NP_112445.1