Queen's Award Received in 2021 ISO 9001 Certified Delivered over 1,000,000 bio-reagents to life science researchers Trusted by Life Science Communities
Cart summary

You have no items in your shopping cart.

Mouse IL1 alpha protein

SKU: orb80049

Description

Mouse IL1 alpha protein

Images & Validation

Tested ApplicationsFA, HPLC, SDS-PAGE
Application Notes
< strong>Experiment Notes: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 1.44 as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using standard solution of IL-1 as Reference Standard.

Key Properties

Biological OriginE.coli
Biological ActivityThe ED50 as determined by the dose-dependant stimulation of murine D10S cells is 0.01 ng/ml, corresponding to Specific Activity of 100,000,000 IU/mg.
TargetMouse IL1 alpha
ConjugationUnconjugated
Purity> 98.0% as determined by RP-HPLC and analysis by SDS-PAGE
Protein SequenceThe sequence of the first five N-terminal amino acids was determined and was found to be Ser-Ala-Pro-Tyr-Thr.

Storage & Handling

StorageStore at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
Buffer/PreservativesThe protein was lyophilized from concentrated (1mg/ml) sterile solution containing 1X PBS, pH=7.4.
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Hematopoietin 1 protein, Hematopoietin-1 protein, IL 1 alpha protein, IL 1 protein, IL 1A protein, IL-1 alpha protein, Il-1a protein, IL1 ALPHA protein, IL1 protein, IL1A protein, IL1A_HUMAN protein, IL1F1 protein, ilia protein, Interleukin 1 alpha protein, Interleukin 1 alpha precursor protein, Interleukin-1 alpha protein, Interleukin1 alpha protein, Preinterleukin 1 alpha protein, Pro interleukin 1 alpha protein

Similar Products

  • Mouse Interleukin 1 Receptor Type I (IL-1R1) ELISA Kit [orb1947472]

    Mouse

    93.75-6000pg/mL

    56.25 pg/mL

    48 T, 96 T
  • Mouse IL1 alpha protein (Active) [orb359005]

    > 97% as determined by SDS-PAGE and HPLC.

    17.9 kDa

    E.Coli

    10 μg, 100 μg, 500 μg
  • Human IL1A Protein, mFc Tag [orb2321373]

    The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.

    The protein has a predicted molecular mass of 44.3 kDa after removal of the signal peptide. The apparent molecular mass of IL1A-mFc is approximately 35-70 kDa due to glycosylation.

    Mammalian

    10 μg, 50 μg, 100 μg
  • Recombinant mouse IL-1 Alpha protein, His (HEK293) [orb1516667]

    > 95% as determined by SDS-PAGE

    24 kDa

    500 μg, 100 μg
  • Mouse IL1 Receptor protein [orb707227]

    ELISA,  MS,  SDS-PAGE,  WB

    Greater than 95% as determined by reducing SDS-PAGE.

    Human cells

    500 μg, 50 μg, 10 μg
Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Protocol Information

Protein Handling and Storage Guide
Protein Handling Guide
View Guide
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
View Protocol
HPLC
High-Performance Liquid Chromatography
View Protocol

Available Sizes

Select a size below

10 μg
$ 340.00
100 μg
$ 1,250.00
500 μg
$ 2,480.00
DispatchUsually dispatched within 2-3 weeks
Bulk Enquiry