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Description
Images & Validation
−| Tested Applications | FA, HPLC, SDS-PAGE |
|---|---|
| Application Notes |
Key Properties
−| Biological Origin | E.coli |
|---|---|
| Biological Activity | The ED50 as determined by the dose-dependant stimulation of murine D10S cells is 0.01 ng/ml, corresponding to Specific Activity of 100,000,000 IU/mg. |
| Target | Mouse IL1 alpha |
| Conjugation | Unconjugated |
| Purity | > 98.0% as determined by RP-HPLC and analysis by SDS-PAGE |
| Protein Sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Ala-Pro-Tyr-Thr. |
Storage & Handling
−| Storage | Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles. |
|---|---|
| Buffer/Preservatives | The protein was lyophilized from concentrated (1mg/ml) sterile solution containing 1X PBS, pH=7.4. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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E.Coli
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The protein has a predicted molecular mass of 44.3 kDa after removal of the signal peptide. The apparent molecular mass of IL1A-mFc is approximately 35-70 kDa due to glycosylation.
Mammalian
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Human cells
500 μg, 50 μg, 10 μg

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