Mouse Med12 Antibody (C-term)

SKU: orb306655

Description

Rabbit polyclonal antibody to Med12

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Product Categories/Products/Antibodies/Primary Antibodies,Epigenetics,Crown Antibodies;Primary Antibodies

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB: 1:2000, WB: 1:1000
• Reactivity: Human, Mouse

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB52354
• Target: Med12
• Molecular Weight: 244561
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti Mediator of RNA polymerase II transcription subunit 12 antibody, anti Mediator complex subunit 12 antibody, anti OPA-containing protein antibody, anti Thyroid hormone receptor-associated protein complex 230 kDa component antibody, anti Trap230 antibody, anti Trinucleotide repeat-containing gene 11 protein antibody, anti Med12 antibody, anti Kiaa0192 antibody, anti Mopa antibody, anti Tnrc11 antibody, anti Trap230 antibody

Anti-Med12 Antibody (C-term) at 1:1000 dilution + mouse spleen lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 245 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-Med12 Antibody (C-term) at 1:1000 dilution + NIH/3T3 whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 245 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-Med12 Antibody (C-term) at 1:2000 dilution. Lane 1: Hela whole cell lysates. Lane 2: C2C12 whole cell lysates. Lane 3: Jurkat whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 245 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Staining Mouse Med12 in mouse stomach sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Quick Database Links

Gene Symbol

Med12

UniProt

A2AGH6