(Mouse) Nr4a2 Antibody (Center)

SKU: orb306502

Description

Rabbit polyclonal antibody to Nr4a2

Research Area

Product Categories/Products/Antibodies,Product Categories/Products/Antibodies/Primary Antibodies,Crown Antibodies;Primary Antibodies

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB: 1:2000
• Reactivity: Mouse, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB52431
• Target: Nr4a2
• Molecular Weight: 66593
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Anti-Nr4a2 Antibody (Center) at 1:2000 dilution + mouse pancreas lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 67 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-Nr4a2 Antibody (Center) at 1:2000 dilution. Lane 1: mouse brain lysates. Lane 2: rat brain lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 67 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Staining (Mouse) Nr4a2 in mouse brain sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Staining (Mouse) Nr4a2 in mouse spleen sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Quick Database Links

Gene Symbol

Nr4a2

UniProt

Q06219