Mouse TNF-alpha Protein, His Tag

SKU: orb1496307

Description

Mouse TNF-alpha, His Tag is expressed from human 293 cells (HEK293). It contains AA Leu 80 - Leu 235 (Accession # P06804).

Research Area

Immunology & Inflammation

Images & Validation

• Application Notes:
  This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 19.1 kDa. The protein migrates as 19-20 kDa and 23-24 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Key Properties

• Expression System: HEK293
• Biological Origin: Mouse
• Biological Activity: Immobilized Mouse TNF-alpha, His Tag at 0.2 µg/mL (100 µL/well) can bind Human TNFR1, Fc Tag with a linear range of 0.1-1 ng/mL (QC tested).
• Target: TNF-alpha
• Reactivity: Mouse
• Tag: C-10×His
• Expression Region: Leu 80 - Leu 235
• Endotoxins: 1.0 EU per μg
• Conjugation: Unconjugated
• MW: 19.1 kDa
• Purity: 95%
• Protein Sequence: Leu 80 - Leu 235

Storage & Handling

• Storage: -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
• Form/Appearance: Powder
• Buffer/Preservatives: PBS, pH 7.4
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Immobilized Mouse TNF-alpha, His Tag (Cat. No. orb1496307) at 0.2 μg/mL (100 μL/well) can bind Human TNFR1, Fc Tag (Cat. No. orb257878) with a linear range of 0.1-1 ng/mL (QC tested).

Quick Database Links

Gene Symbol

TNF-alpha

UniProt

P06804