Murine BD2 protein

SKU: orb82605

Description

Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The alpha-defensins are distinguished from the beta-defensins by the pairing of their three disulfide bonds. To date, four beta-defensins have been identified; BD1, BD2, BD3 and BD4. beta-defensins are expressed on some leukocytes and at epithelial surfaces. In addition to their direct antimicrobial activities, they are chemoattractant towards immature dendritic cells and memory T cells. The beta-defensin proteins are expressed as the C-terminal portion of precursors and are released by proteolytic cleavage of a signal sequence and, in the case of BD1 (36 a.a.), a propeptide region. beta-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds.

Images & Validation

• Tested Applications: HPLC, SDS-PAGE
• Application Notes:
  Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Further dilutions should be made in appropriate buffered solutions.

Key Properties

• Biological Origin: E.coli
• Biological Activity: Determined by its ability to chemoattract immature human dendritic cells using a concentration of 10.0-100.0 ng/ml. Less than 1EU/μg of rMuBD-2 as determined by LAL method.
• Target: BD2
• Conjugation: Unconjugated
• MW: 5.54 kDa
• Purity: > 98% pure by SDS-PAGE and HPLC analyses.
• Protein Sequence: AVGSLKSIGY EAELDHCHT N GGYCVRAICP PSARRPGSCF PEKNPCCKYM K

Storage & Handling

• Storage: Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
• Buffer/Preservatives: Sterile Filtered White lyophilized (freeze-dried) powder. We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

SDS-PAGE analysis of Murine BD2 protein

Quick Database Links

Gene Symbol

BD2