MYOC monoclonal antibody (M01), clone 4F8

SKU: orb2292543

Description

Mouse monoclonal antibody raised against a partial recombinant MYOC.

Images & Validation

• Tested Applications: ELISA, IHC-P, IP, WB
• Reactivity: Human

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1 Kappa
• Clone No.: 4F8
• Immunogen: MYOC (AAH29261, 36 a.a. ~ 135 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Protein Sequence: QLRKANDQSGRCQYTFSVASPNESSCPEQSQAMSVIHNLQRDSSTQRLDLEATKARLSSLESLLHQLTLDQAARPQETQEGLQRELGTLRRERDQLETQT
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: In 1x PBS, pH 7.4
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Detection limit for recombinant GST tagged MYOC is approximately 0.3 ng/ml as a capture antibody.

Immunoperoxidase of monoclonal antibody to MYOC on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml]

Immunoprecipitation of MYOC transfected lysate using anti-MYOC monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MYOC MaxPab rabbit polyclonal antibody.

Western Blot analysis of MYOC expression in transfected 293T cell line by MYOC monoclonal antibody (M01), clone 4F8. Lane 1: MYOC transfected lysate (57 KDa). Lane 2: Non-transfected lysate.

Western blot analysis of MYOC over-expressed 293 cell line, cotransfected with MYOC Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with MYOC monoclonal antibody (M01), clone 4F8 (Cat # orb2292543). GAPDH (36.1 kDa) used as specificity and loading control.

Western Blot detection against Immunogen (36.63 KDa).

Quick Database Links

RefSeq:AAH29261