NANOG Mouse Monoclonal Antibody

SKU: orb783390

Description

NANOG Mouse Monoclonal Antibody

Research Area

Cell Differentiation, Cell Biology, Intercellular Signaling, Cell Signaling, Intracellular Signaling Proteins, Cell Signaling, Surface Molecules, Molecular Biology, Transcription Factors, DNA and RNA Research

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:400-800, IHC-F=1:400-800, IF=1:100-500
• Reactivity: Human
• Predicted Reactivity: Other

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone No.: N3F1
• Immunogen: Recombinant human NANOG.
• Target: NANOG
• Molecular Weight: 36 kDa
• Purification: affinity purified by Protein G
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NANOG_MOUSE; Embryonic stem cell specific homeobox protein(Nanog); ENK; FLJ12581; FLJ40451; Homeobox transcription factor Nanog; Nanog homeobox; Homeobox protein NANOG; ES cell-associated protein 4; Early embryo specific expression NK-type homeobox protein; Homeobox transcription factor Nanog; Nanog; Ecat4.

Paraformaldehyde-fixed, paraffin embedded (human testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (NANOG) Monoclonal Antibody, Unconjugated (orb783390) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Sample: Lane 1: K562 (Human) Cell Lysate, Primary: Anti-NANOG (orb783390) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 36 kD, Observed band size: 36 kD.

Sample: Lane 1: NANOG Recombinant protein, Primary: Anti-NANOG (orb783390) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 36 kD, Observed band size: 45 kD.

Quick Database Links

Gene Symbol

NANOG

UniProt

Q9H9S0