NECAB1 Antibody / N-terminal EF-hand calcium binding protein 1

SKU: orb1822858

Description

Members of the EF-CBP (N-terminal EF-hand calcium binding protein)/NECAB (neuronal calcium-binding protein) family participate in neuronal calcium signaling. EF-CBP1 (N-terminal EF-hand calcium binding protein 1), also known as STIP-1 or neuronal calcium-binding protein 1 (NECAB1), is a 351 amino acid cytoplasmic protein that contains one antibiotic biosynthesis monooxygenase (ABM) domain and 2 EF-hand domains. Expressed in brain, EF-CBP1 interacts with copine 6 and Syntaxin, and exists as two alternatively spliced isoforms. The gene encoding EF-CBP1 maps to human chromosome 8, which consists of nearly 146 million base pairs, encodes over 800 genes and is associated with a variety of diseases and malignancies. Schizophrenia, bipolar disorder, Trisomy 8, Pfeiffer syndrome, congenital hypothyroidism, Waardenburg syndrome and some leukemias and lymphomas are thought to occur as a result of defects in specific genes that map to chromosome 8.

Images & Validation

• Tested Applications: IHC-P
• Dilution Range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG
• Clone No.: NECAB1/7677
• Immunogen: Recombinant human NECAB1 protein was used as the immunogen for the NECAB1 antibody.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Aliquot the NECAB1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Expiration Date: 12 months from date of receipt.
• Hazard Information: This NECAB1 antibody is available for research use only.
• Disclaimer: For research use only

IHC staining of FFPE human brain tissue with NECAB1 antibody (clone NECAB1/7677). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human brain tissue with NECAB1 antibody (clone NECAB1/7677). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free NECAB1 antibody (clone NECAB1/7677) as confirmation of integrity and purity.

Quick Database Links

UniProt

Q8N987