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NEFL
Description
Research Area
Images & Validation
−| Tested Applications | FC, ICC, IHC, WB |
|---|---|
| Dilution Range | WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; ICC: 1/200 - 1/1000; FCM: 1/200 - 1/400; ELISA: 1/10000 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Clone No. | 1H3A4 |
| Immunogen | Purified recombinant fragment of human NEFL expressed in E. Coli. |
| Target | NEFL |
| Molecular Weight | 62kDa |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Ascitic fluid containing 0.03% sodium azide |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Confocal immunofluorescence analysis of Hela cells using NEFL antibody

Line graph illustrates about the Ag-Ab reactions using different concentrations of antigen and serial dilutions of NEFL antibody

Western blot analysis of Hela (Lane1) and Jurkat (Lane2) cell lysate using NEFL antibody

Western blot analysis of human NEFL (AA: 422-543) recombinant protein using NEFL antibody

Immunohistochemical analysis of formalin fixed and paraffin-embedded lung cancer tissues using NEFL antibody

Flow cytometric analysis of Jurkat cells using NEFL antibody

IHC-P of bladder cancer tissues using NEFL antibody
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Protocol Information
NEFL (orb97415)
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