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Description
Research Area
Images & Validation
−| Tested Applications | ELISA, ICC, IF, IHC-P |
|---|---|
| Dilution Range | IF/ICC: 1:200, IHC-P: 1:200 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human Nestin. Please contact us for the exact immunogen sequence. The peptide is available as orb374873. |
| Target | Nestin |
| Molecular Weight | 178 kDa |
| Purity | Polyclonal antibodies are purified by peptide affinity chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | 10 mM PBS, 0.02% sodium azide |
| Concentration | - 100 μg (in 200 μl): 0.5 mg/ml- 200 μg (in 400 μl): 0.5 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Mouse anti Nestin [orb98393]
ICC, IHC-Fr, IHC-P, WB
Human, Monkey, Primate
Mouse
Monoclonal
Unconjugated
0.1 mg

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IHC-P staining of mouse kidney tissue using anti-Nestin (2.5 ug/ml)

Immunofluorescence analysis of rat brain tissue using anti-Nestin (2.5 ug/ml)

Immunohistochemical staining of rat brain tissue using Nestin antibody (2.5 ug/ml)

Immunohistochemical staining of mouse kidney tissue using Nestin antibody (2.5 ug/ml)

IHC-P image of rat brain tissue using anti-Nestin (2.5 ug/ml)

Immunofluorescence image of rat brain tissue using anti-Nestin (2.5 ug/ml)
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Protocol Information
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Seyed Majid Jalalian Hosseini 1 , Javad Baharara2 *, Shahrbanoo Oryan3 and Mohammad Amin Kerachian Combined Effect of Decellularized Rat Brain Tissue and Nerve Growth Factor on 5 Neural Differentiation of PC12 Cell Line in 2D Cell Culture Pakistan J. Zool, (2024)
Applications
Reactivity
Nestin antibody (orb251614)
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