Nestin Mouse Monoclonal Antibody

SKU: orb1816769

Description

Nestin Mouse Monoclonal Antibody

Research Area

,Angiogenesis,Tumor antigens,Angiogenic Factors,Ectoderm,Intermediate Filaments,Neural Stem Cell marker,Neurogenesis,nestin,Ectoderm,Intracellular,Neuron Restricted Lineage

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: IHC-P=1:100-500, WB=1:500-2000, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human
• Predicted Reactivity: Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a κ
• Clone No.: E2G4
• Immunogen: KLH conjugated synthetic peptide derived from human Nestin
• Target: NES
• Molecular Weight: 178 kDa
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS, Glycerol, rAlbumin.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ESTM 46; FLJ 21841; FLJ21841; Intermediate filament protein; Nbla00170; Nes; NEST_HUMAN; Nestin.

Paraformaldehyde-fixed, paraffin embedded (human brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (Nestin) Monoclonal Antibody, Unconjugated (orb1816769) at 1:500 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (Nestin) Monoclonal Antibody, Unconjugated (orb1816769) at 1:500 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human spleen), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (Nestin) Monoclonal Antibody, Unconjugated (orb1816769) at 1:500 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Quick Database Links

Gene Symbol

NES

UniProt

P48681