Neurofilament, phospho (NF-H) (Neuronal Marker) Antibody

SKU: orb2318181

Description

Neurofilament, phospho (NF-H) (Neuronal Marker) Antibody

Research Area

,Cardiovascular|Neuroscience

Images & Validation

• Tested Applications: FC, IHC, WB
• Dilution Range: Western Blot (1-2ug/ml); Flow Cytometry (1-2ug/million cells); Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.
• Reactivity: Bovine, Feline, Gallus, Guinea pig, Human, Mouse, Porcine, Rabbit, Rat, Rodent
• Application Notes:
  Positive Control: HEK293 cells, Brain, Neuroblastoma.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: NE14
• Immunogen: Crude neurofilament preparation from porcine spinal cord
• Target: NEFH
• Source/Expression System: HEK293 cells
• Molecular Weight: 200kDa
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Concentration: Purified Ab with BSA and Azide at 200ug/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NEFH; Neurofilament H; Neurofilament Heavy Polypeptide 200kDa; Neurofilament Triplet H Protein; NF-H; NF200

Quick Database Links

Gene Symbol

NEFH

UniProt

P12036

NCBI Gene ID

4744