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Description
Research Area
Images & Validation
−| Tested Applications | ICC, IF, IHC-Fr, IHC-P, IP, WB |
|---|---|
| Dilution Range | WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone No. | 3D2-4E9-7A8 |
| Immunogen | Recombinant Protein of Transcription factor p65 |
| Target | RELA |
| Molecular Weight | Calculated MW: 60 kDa; Observed MW: 65 kDa |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3. |
| Concentration | 1.43 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Western blot analysis of NF-KB p65 in T47D, 3T3, COS7, C6 and Hela lysates using NF-KB p65 antibody.

Immunofluorescence analysis of NF-KB p65 (3D2) in Hela using NF-KB p65 (3D2) antibody, and DAPI (blue).

Immunoprecipitation analysis of NF-KB p65 (3D2) in Hela lysates using NFκB p65 (3D2) antibody.

Immunohistochemistry analysis of paraffin-embedded rat Brain Tissue using NF-KB p65 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Western blot analysis of NF-KB p65 (3D2) in COS7, C6, T47D lysates using NF-KB p65 (3D2) antibody.
Quick Database Links
UniProt Details
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Protocol Information
NF-KB p65 (3D2) Mouse mAb (orb1563250)
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