Nitrotyrosine Antibody: APC

SKU: orb147397

Description

Mouse monoclonal to Nitrotyrosine (APC). Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an important component of nitric oxide signaling. While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosino-philperoxidase, myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase. Nitrotyrosine may also serve as a biomarker for the effects of reactive nitrogen oxides, based on tyrosine residues becoming nitrated in proteins at sites of inflammation induced tissue injury. The presence of nitro tyrosine-containing proteins therefore has shown high correlation to disease states such as atherosclerosis, Alzheimers disease, Parkinsons disease and amyotrophic lateral sclerosis..

Research Area

Product Categories/Products/Antibodies,Product Categories/Products/Antibodies/Primary Antibodies,Cancer | Oxidative Stress | Cell Signaling | Post-translational Modifications | Nitration | Neuroscience | Neurodegeneration | Alzheimer's Disease | Parkinson's Disease

Images & Validation

• Tested Applications: ICC, IF, IHC
• Dilution Range: WB (1:1400), IHC (1:100)
• Reactivity: All
• Application Notes:
  0.7 µg/ml of SMC-154 was sufficient for detection of 5 µg SIN-1 treated BSA by Western Blot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone No.: 39B6
• Immunogen: 3-(4-hydroxy-3-nitrophenylacetamido) propionic acid-bovine serum albumin
• Target: Nitrotyrosine
• Conjugation: APC

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 95.64mM Phosphate, 2.48mM MES and 2mM EDTA
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Nitro tyrosine antibody, 3-Nitrotyrosine antibody

Quick Database Links

Gene Symbol

Nitrotyrosine