Nitrotyrosine Antibody: Biotin

SKU: orb147398

Description

Mouse monoclonal to Nitrotyrosine (Biotin). Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an important component of nitric oxide signaling. While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosino-philperoxidase, myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase. Nitrotyrosine may also serve as a biomarker for the effects of reactive nitrogen oxides, based on tyrosine residues becoming nitrated in proteins at sites of inflammation induced tissue injury. The presence of nitro tyrosine-containing proteins therefore has shown high correlation to disease states such as atherosclerosis, Alzheimers disease, Parkinsons disease and amyotrophic lateral sclerosis..

Research Area

Images & Validation

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Item 1 of 8

Tested Applications	ELISA, ICC, IF, IHC, WB
Dilution Range	WB (1:1400), IHC (1:100)
Reactivity	All
Application Notes	0.7 µg/ml of SMC-154 was sufficient for detection of 5 µg SIN-1 treated BSA by Western Blot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

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Host	Mouse
Clonality	Monoclonal
Isotype	IgG2a
Clone No.	39B6
Immunogen	3-(4-hydroxy-3-nitrophenylacetamido) propionic acid-bovine serum albumin
Target	Nitrotyrosine
Conjugation	Biotin

Storage & Handling

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Storage	Conjugated antibodies should be stored according to the product label
Buffer/Preservatives	136.36mM Ethanolamine, and 9.55mM Sodium Bicarbonate in 95.45% PBS
Concentration	1 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Nitro tyrosine antibody, 3-Nitrotyrosine antibody

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