Notch1 Antibody: HRP

SKU: orb150339

Description

Mouse monoclonal to Notch1 (HRP). Notch is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane..

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Cancer,Cardiovascular Disease,Neuroscience,Epigenetics,Developmental Biology,Neuroscience | Cell Signaling | Epigenetics and Nuclear Signaling | cardiovascular System | Heart

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: WB (1:1000)
• Reactivity: Human, Mouse, Rat
• Application Notes:
  1 µg/ml of SMC-430 was sufficient for detection of Notch1 in 20 µg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: N253/32 (Formerly sold as S253-32)
• Immunogen: Fusion protein amino acids 20-216 (extracellular N-terminus, EGF-like domains 1-5) of mouse Notch1
• Target: Notch1
• Molecular Weight: 270kDa
• Conjugation: HRP

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide and 0.23mM Citrate in dH2O
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Neurogenic locus notch homolog protein 1 antibody, hN1 antibody, Translocation associated notch protein antibody, TAN1 antibody, TAN-1 antibody, Motch A antibody, mT14 antibody, p300 antibody, Motch antibody, Mis6 antibody, Neurogenic locus notch protein homolog antibody, NICD antibody, Notch 1 intracellular domain antibody, Notch gene homolog 1 (Drosophila) antibody, Notch homolog 1 antibody, translocation-associated (Drosophila) antibody, NOTCH Drosophila antibody, homolog of 1 antibody, Translocation associated notch homolog antibody, Translocation associated notch protein TAN 1 antibody, xotch antibody

Western Blot analysis of Rat Brain Membrane showing detection of ~270 kDa Notch1 protein using Mouse Anti-Notch1 Monoclonal Antibody, Clone N253/32. Lane 1: MW Ladder. Lane 2: Rat Brain Membrane (10 μg). Load: 10 μg. Block: 5% milk. Primary Antibody: Mouse Anti-Notch1 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: ~270 kDa.

Quick Database Links

Gene Symbol

Notch1

UniProt

Q01705

NCBI Gene ID

18128

RefSeq (Protein):NP_032740.3