NPTX1-Y344 Antibody

SKU: orb37707

Description

Peptide affinity purified rabbit polyclonal antibody.

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Product Categories/Products/Antibodies/Primary Antibodies,Epigenetics,Primary Antibodies;Neuroscience;SARS CoV2 PPIs

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB: 1:1000, IHC-P: 1:50-100
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB15369
• Immunogen: Synthetic Peptide
• Target: NPTX1
• Molecular Weight: 47122
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-NP1 antibody, anti-Neuronal pentraxin I antibody, anti-NP-I antibody, anti-47 kDa taipoxin-binding protein antibody

Western blot analysis of NPTX1-Y344 in mouse brain tissue lysates (35 ug/lane). NPTX1 (arrow) was detected using the purified Pab.

All lanes: Anti-NPTX1Y344 Antibody at 1:2000 dilution. Lane 1: human brain lysate. Lane 2: mouse brain lysate. Lane 3: rat brain lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 47 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-NPTX1-Y344 Antibody at 1:2000 dilution. Lane 1: human brain lysate. Lane 2: mouse brain lysate. Lane 3: rat brain lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 47 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-NPTX1-Y344 Antibody at 1:2000 dilution. Lane 1: human brain lysate. Lane 2: mouse brain lysate. Lane 3: rat brain lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 47 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-NPTX1-Y344 Antibody at 1:2000 dilution. Lane 1: human brain lysate. Lane 2: mouse brain lysate. Lane 3: rat brain lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 47 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Staining NPTX1 in human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Staining NPTX1 in human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Quick Database Links

Gene Symbol

NPTX1

UniProt

Q15818

RefSeq (Protein):NP_002513.2