NQO1 Antibody

SKU: orb1247187

Description

NQO1 Antibody

Research Area

,Cancer

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Reactivity: Human, Porcine, Rat
• Predicted Reactivity: Canine
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 28kDa band observed in lysates of Glioblastoma cell line U251 and approx 30kDa band observed in Human Kidney and Lung lysates and in Rat and Pig Kidney lysates (calculated MW of 27.3kDa according to Human NP_001020604.1 and 30.9kDa according to Human NP_000894.1, Rat NP_058696.2 and Pig NP_001153085.1). Recommended concentration: 0.03-0.3ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:Paraffin embedded Human Kidney. Recommended concentration: 5ug/ml.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HepG2 and U251 cells. Recommended concentration: 5-10ug/ml.

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Key Properties

• Antibody Type: Primary Antibody
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-SIPTDNQIKARK
• Target: NQO1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NQO1, NAD(P)H dehydrogenase, quinone 1, DTD, QR1, DHQU, DIA4, NMOR1, NMORI, diaphorase-4, diaphorase (NADH/NADPH), diaphorase (NADH/NADPH) (cytochrome b-5 reductase), NAD(P)H:menadione oxidoreductase 1, dioxin-inducible 1, DT-diaphorase, NAD(P)H menadione oxidoreductase 1, dioxin-inducible, NAD(P)H:Quinone acceptor oxidoreductase type 1, NAD(P)H menadione oxidoreductase 1, dioxin-inducible, NAD(P)H:quinone oxireductase, azoreductase, diaphorase (NADH/NADPH) (cytochrome b-5 reductase), dioxin-inducible 1, menadione reductase, phylloquinone reductase, quinone reductase 1

Quick Database Links

Gene Symbol

NQO1

UniProt

P15559

RefSeq (Protein):NP_001020605.1, NP_000894.1, NP_001020604.1