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NR2C2 Antibody

SKU: orb1265587

Description

NR2C2 Antibody

Research Area

,Other

Images & Validation

Tested ApplicationsIF, WB
ReactivityHuman
Predicted ReactivityMouse, Rat
Application Notes
For WB starting dilution is: 1:1000For IF starting dilution is: 1:10~50

Key Properties

Antibody TypePrimary Antibody
ClonalityPolyclonal
IsotypeRabbit Ig
ImmunogenThis NR2C2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 438-466 amino acids from the C-terminal region of human NR2C2.
TargetNR2C2
Molecular Weight65 kDa
PurificationThis antibody is purified through a protein A column, followed by peptide affinity purification.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSupplied in PBS with 0.09% (W/V) sodium azide.
Concentrationbatch dependent
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Nuclear receptor subfamily 2 group C member 2, Orphan nuclear receptor TAK1, Orphan nuclear receptor TR4, Testicular receptor 4, NR2C2, TAK1, TR4

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NR2C2 Antibody

Western blot analysis in Jurkat cell line lysates (35 ug/lane).

NR2C2 Antibody

Fluorescent confocal image of Hela cell stained with NR2C2 Antibody. Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NR2C2 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). NR2C2 immunoreactivity is localized to Nucleus significantly.

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product
RefSeqP49116

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IF
Immunofluorescence
View Protocol

NR2C2 Antibody (orb1265587)

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Available Sizes

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400 μl
$ 880.00
Free Secondary Antibody (20 ul)0/0

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