NSE Antibody / Neuron Specific Enolase

SKU: orb1825910

Description

This monoclonal antibody recognizes a protein of about 50kDa, which is identified as gamma-enolase. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue whereas gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE-gamma is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas.It it be usually employed in combination with other markers such as Synaptophysin,Chromogranin A, and Neurofilament.

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2b, kappa
• Clone No.: ENO2/4507
• Immunogen: A recombinant fragment of human NSE gamma (within amino acids 416-433) was used as the immunogen for the Gamma Enolase antibody.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Aliquot the Gamma Enolase antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Expiration Date: 12 months from date of receipt.
• Hazard Information: This Gamma Enolase antibody is available for research use only.
• Disclaimer: For research use only

Quick Database Links

UniProt

P09104