NSUN2 Antibody

SKU: orb1257870

Description

NSUN2 Antibody

Research Area

,Other

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 508-767 of human NSUN2 (NP_060225.4).
• Target: NSUN2
• Molecular Weight: Observed: 105kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NSUN2, FLJ20303, MISU, SAKI, TRM4, MRT5

Western blot analysis of extracts of various cell lines, using NSUN2 antibody (orb1257870) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunofluorescence analysis of C6 cells using NSUN2 antibody (orb1257870) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U2OS cells using NSUN2 antibody (orb1257870) at dilution of 1:100. Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

NSUN2

UniProt

Q08J23