Nuclease

SKU: orb257222

Description

Nuclease is a recombinant form of Serratia marcescens extracellular endonuclease produced in Escherichia coli cells. Nuclease is a homodimer with monomer molecular masses about 30 kDa. Two disulfide bonds found in the nuclease are crucial to its activity and stability. The enzyme is a non-specific nuclease with high specific activity, which degrades both single- and double-stranded nucleic acids in any form (single stranded, double stranded, linear, circular and supercoiled). It hydrolyzes internal phosphodiester bonds present between the nucleotides to 5‘- phosphorylated oligonucleotides of 3-5 bases in length.

Images & Validation

• Application Notes:
  BenzNuclease is a recombinant form of Serratia macescens extracellular endonuclease produced in Escherichia coli cells using a proprietary process. BenzNuclease is a homodimer with monomer molecular masses about 30 kDa. Two disulfide bonds found in the nuclease are crucial to its activity and stability. The enzyme is a non-specific nuclease with high specific activity, which degrades both single- and double-stranded nucleic acids in any form ( single stranded, double stranded, linear, circular and supercoiled ). It hydrolyzes internal phosphodiester bonds present between the nucleotides to 5'-phosphorylated oligonucleotides of 3-8 bases in length.

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Key Properties

• Expression System: E. coli
• Biological Origin: Serratia marcescens
• Target: Nuclease
• Reactivity: Bacteria
• Tag: Native
• Endotoxins: 1.0 EU per μg
• Conjugation: Unconjugated
• MW: 26.8 kDa
• Purity: 95%

Storage & Handling

• Storage: Avoid repeated freeze-thaw cycles. This product is stable after storage at:. In lyophilized state for 1 year (-20°C); After reconstitution under sterile conditions for 3 months (-70°C)
• Form/Appearance: Powder
• Buffer/Preservatives: 20mM Tris, 20mM NaCl, 2mM MgCl2, pH 8.0
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

SDS-PAGE analysis of BenzNuclease protein

Quick Database Links

Gene Symbol

Nuclease