Oct4 Antibody / POU5F1

SKU: orb1716299

Description

OCT4/POU5F1 is a member of the POU family of transcription factors, involved in the regulation of pluripotency during normal development and is detectable in embryonic stem and germ cells. It is a key regulator of self-renewal in embryonic stem cells; its expression is potentially correlated with tumorigenesis and can affect tumor recurrence or resistance to therapies. OCT4/POU5F1 is expressed in undifferentiated pluripotent cells, germ cells in ovary and testis.

Images & Validation

• Tested Applications: IHC-P
• Dilution Range: Immunohistochemistry (FFPE): 1-2ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the POU5F1 antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: Rabbit IgG, kappa
• Clone No.: OCT4/6847R
• Immunogen: Recombinant fragment from the human protein was used as the immunogen for the POU5F1 antibody.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Aliquot the POU5F1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Expiration Date: 12 months from date of receipt.
• Hazard Information: This POU5F1 antibody is available for research use only.
• Disclaimer: For research use only

IHC staining of FFPE human seminoma tissue with OCT4 antibody (clone OCT4/6847R) at 2 ug/ml. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human seminoma tissue with OCT4 antibody (clone OCT4/6847R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Quick Database Links

UniProt

Q01860