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Description
Research Area
,Product Categories/Antibodies
Images & Validation
−Item 1 of 4
| Tested Applications | ELISA, IHC-P, WB |
|---|---|
| Dilution Range | P-ELISA: 1:500-1000, WB: 1:100-500, IHC-P: 1:100-500 (based on 0.5 mg/ml) |
| Reactivity | Bacteria |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human ompF. Please contact us for the exact immunogen sequence. The peptide is available as orb73071. |
| Target | ompF |
| Molecular Weight | 42 kDa |
| Purity | Polyclonal antibodies are purified by peptide affinity chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles. |
|---|---|
| Form/Appearance | 10 mM PBS, 0.02% sodium azide |
| Concentration | - 100 μg (in 200 μl): 0.5 mg/ml- 200 μg (in 400 μl): 0.5 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−anti-ompF antibody
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10% SDS- PAGE followed by a Western blot: lane 1, ladder; lanes 2
3&4, wild type( BW 25113) + kanamycin (0xMIC, ½x MIC and 3xMIC respectively); Lanes 5
6&7, ΔOmpF (JW0912) + kanamycin (0xMIC, ½x MIC and 3xMIC respectively).
Lane 1, ladder; lanes 2
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
ompF antibody (orb13626)
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