OSGIN1 Rabbit Polyclonal Antibody

SKU: orb100666

Description

OSGIN1 Rabbit Polyclonal Antibody

Research Area

Cell Differentiation, Cell Biology, Oxidative Stress

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Equine

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human OSGIN1 (351-450/560aa)
• Target: OSGIN1
• Molecular Weight: 61 kDa
• Purification: affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

BDGI; Bone marrow stromal cell derived growth inhibitor; huOKL38; OKL 38; OKL38; OSGIN 1; Ovary kidney and liver protein 38; Oxidative stress induced growth inhibitor 1; Pregnancy induced growth inhibitor; Pregnancy induced growth inhibitor OKL38; OSGI1_HUMAN.

Paraformaldehyde-fixed, paraffin embedded (rat ovary), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (OSGIN1) Polyclonal Antibody, Unconjugated (orb100666) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Human HepG2 cell lysates, Lane 2: Human MCF-7 cell lysates, Primary: Anti-OSGIN1 (orb100666) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 61 kDa, Observed band size: 61 kDa.

Sample: Liver (Mouse) Lysate at 40 ug, Primary: Anti-OSGIN1 (orb100666) at 1/300 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1/5000 dilution, Predicted band size: 61 kD, Observed band size: 55 kD.

Sample: MCF-7 Cell (Human) Lysate at 40 ug, Primary: Anti-OSGIN1 (orb100666) at 1/300 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1/5000 dilution, Predicted band size: 61 kD, Observed band size: 61 kD.

Quick Database Links

Gene Symbol

OSGIN1

UniProt

Q9UJX0