p38 Antibody: HRP

SKU: orb151881

Description

Rabbit polyclonal to p38 (HRP). The MAPK (mitogen activated protein kinase) comprises a family of ubiquitous praline-directed, protein-serine/threonine kinases which signal transduction pathways that control intracellular events including acute responses to hormones and major developmental changes in organisms. This super family consists of stress activated protein kinases (SAPKs); extracellular signal-regulated kinases (ERKs); and p38 kinases, each of which forms a separate pathway. The kinase members that populate each pathway are sequentially activated by phosphorylation. Upon activation, p38 MAPK/SAPK2alpha translocates into the nucleus where it phosphorylates one or more nuclear substrates, effecting transcriptional changes and other cellular processes involved in cell growth, division, differentiation, inflammation, and death. Specifically p38 always acts as a pro-apoptotic factor with its activation leading to the release of cytochrome c from mitochondria and cleavage of caspase 3 and its downstream effector, PARP. p38 MAPK is activated by a variety of chemical stress inducers including hydrogen peroxide, heavy metals, anisomycin, sodium salicylate, LPS, and biological stress signals such as tumor necrosis factor, interleukin-1, ionizing and UV irradiation, hyperosmotic stress and chemotherapeutic drugs. As a result, p38 alpha has been widely validated as a target for inflammatory disease including rheumatoid arthritis, COPD and psoriasis and has also been implicated in cancer, CNS and diabetes..

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Cancer,Neuroscience,Signaling Pathways,Product Categories/Products/Antibodies/Primary Antibodies,Signaling Pathways/Apoptotic,Epigenetics,Cell Signaling | Post-translational Modifications | Phosphorylation | Cancer

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: WB (1:1000), ICC/IF (1:100), IP (1:250)
• Reactivity: Bovine, Canine, Gallus, Guinea pig, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep
• Application Notes:
  A 1:1000 dilution of SPC-172 was sufficient for detection of p38 in 20 µg of HeLa cell lysate by ECL immunoblot analysis.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: A 20 residue synthetic peptide based on the human p38 with the cysteine residue added and coupled to KLH
• Target: p38
• Molecular Weight: 43kDa
• Conjugation: HRP

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide and 0.23mM Citrate in dH2O
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CSAID Binding protein 1 antibody, CSBP1 antibody, CSBP2 antibody, EXIP antibody, MAP kinase MXI2 antibody, MAPkinase p38alpha antibody, MAPK14 antibody, p38 ALPHA antibody, p38 MAP kinase antibody, p38 mitogen activated protein kinase antibody, RK antibody, SAPK 2A antibody, Stress activated protein kinase 2A antibody

Quick Database Links

Gene Symbol

p38

UniProt

Q16539

NCBI Gene ID

1432

RefSeq (Protein):NP_001306.1