p53 Antibody (C-term)

SKU: orb1930145

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

,Primary Antibodies;Antibody Collections;Cancer;Cell Biology;Microbiology;Signal Transduction;Amyotrophic Lateral Sclerosis (ALS);Anti-Wnt-Pathway (plus Lgr5) Antibodies;Viral process;Host-virus interaction;Apoptosis;Apoptosis signaling;Mitochondrion;Necroptosis;Necrosis;SARS-Human interaction partners

Images & Validation

• Tested Applications: WB
• Dilution Range: WB: 1:1000-1:2000, WB: 1:2000
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB07832
• Target: This p53 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 354-385 amino acids from the C-terminal region of human p53.
• Molecular Weight: 43653 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53, TP53, P53

All lanes: Anti-p53 Antibody (C-term) at 1:2000 dilution. Lane 1: 293 whole cell lysate. Lane 2: HT-29 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 44 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-p53 Antibody (S376) (p53-S378) at 1:1000-1:2000 dilution. Lane 1: 293T/17 whole cell lysate. Lane 2: A431 whole cell lysate. Lane 3: SK-BR-3 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 44 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

This p53 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 354-385 amino acids from the C-terminal region of human p53.

UniProt

P04637

RefSeq (Protein):NP_000537.3, NP_00126, NP_001263624.1, NP_001263625.1, NP_001119584.1, NP_001119586.1, NP_001119587.1, NP_001119589.1, NP_001263627.1, NP_001263628.1, NP_001263626.1, NP_001119590.1, NP_001119588.1, NP_001119585.1