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Description
Research Area
,Apoptosis,Cancer,Cell Cycle,Neuroscience,Signal Transduction,Transcription
Images & Validation
−Item 1 of 5
| Tested Applications | IF, IHC, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant protein of human p53 |
| Target | TP53 |
| Molecular Weight | Observed: 53kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Liquid |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−TP53, P53, BCC7, LFS1, TRP53
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Western blot analysis of extracts of various cell lines, using p53 antibody (orb1257920) at 1:300 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human gastric cancer using p53 Antibody (orb1257920) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human B cell lymphoma using p53 Antibody (orb1257920) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using p53 Antibody (orb1257920) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF-7 cells using p53 antibody (orb1257920).
Quick Database Links
Gene Symbol
TP53
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
p53 Antibody (orb1257920)
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