p53 Tumor Suppressor Protein Antibody

SKU: orb2317430

Description

p53 Tumor Suppressor Protein Antibody

Research Area

,AKT Signaling|Apoptosis|Bladder Cancer|Breast Cancer|Cancer|Cardiovascular|Colon Cancer|Cytokine Signaling|Defective Intrinsic Apoptosis|Hypoxia|Immunology|Infectious Disease|Lung Cancer|MAPK Signaling|Nuclear Marker|Ovarian Cancer|Signal Transduction|Transcription Factors

Images & Validation

• Tested Applications: FC, IF, IHC, WB
• Dilution Range: Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); Western Blot (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.
• Reactivity: Human
• Application Notes:
  Positive Control: MDA-MB-231 cells. Breast or Colon carcinoma

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant full-length human TP53 protein
• Target: TP53
• Molecular Weight: 53kDa.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 200ug/ml of Ab purified from rabbit anti-serum by Protein A. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin at 1.0mg/ml.
• Concentration: Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Antigen NY-CO-13, BCC7, Cellular Tumor Antigen p53, LFS1, TP53, Transformation Related Protein 53 (TRP53), Tumor Protein p53, Tumor Suppressor p53

Quick Database Links

Gene Symbol

TP53

UniProt

P04637

NCBI Gene ID

7157