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Description
Research Area
Images & Validation
−| Tested Applications | ELISA, IF, IHC-P, WB |
|---|---|
| Dilution Range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human PAK1. AA range:178-227 |
| Molecular Weight | 60 |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Western Blot analysis of various cells using PAKα Polyclonal Antibody diluted at 1:500.

Immunofluorescence analysis of HeLa cells, using PAK1 Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using PAK1 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from 293 cells, treated with Etoposide 25uM 60', using PAK1 Antibody. The lane on the right is blocked with the synthesized peptide.
Protocol Information
PAKα rabbit pAb (orb766021)
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