Pan-Cytokeratin, 2 Clones (AE1/AE3)

SKU: orb27182

Description

Mouse monoclonal antibody to pan Cytokeratin

Images & Validation

• Tested Applications: IHC-Fr, IHC-P
• Dilution Range: (liquid conc.) 1:20 - 1:50
• Reactivity: Human
• Application Notes:
  Positive Control: Appendix. Pre-Treatment: Paraffin sections may be pre-treated with 1 % protease solution (Unmasking fluid P, Art.-No. DE110) for 15 min at RT, improvement of staining may be achieved with Unmasking Fluid G (Art. No. DE007) or Unmasking Fluid C (Art. No. DE000). Incubation Time: 60 min at RT

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: AE1/AE3
• Immunogen: Human epithelial keratins
• Purification: Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 200 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art .No. PU002.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 200 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art .No. PU002.
• Concentration: 70 µg/ml
• Expiration Date: 12 months from date of receipt.
• Hazard Information: *These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
• Disclaimer: For research use only

Immunohistochemical staining of human epidermis using pan Cytokeratin antibody