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Description
Research Area
Images & Validation
−| Tested Applications | IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution Range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Bovine, Canine, Equine, Porcine, Rabbit |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human pan-cytokeratin (331-430/590aa) |
| Purification | affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Preservative: 0.02% Proclin300, Constituents: 1% rAlbumin, 0.01M PBS, pH 7.4. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Sample: Lane 1: Skin (Mouse) Lysate at 40 ug, Lane 2: Uterus (Mouse) Lysate at 40 ug, Lane 3: Urinary bladder (Mouse) Lysate at 40 ug, Lane 4: Esophagus (Mouse) Lysate at 40 ug, Lane 5: Skin (Rat) Lysate at 40 ug, Lane 6: Uterus (Rat) Lysate at 40 ug, Lane 7: Urinary bladder (Rat) Lysate at 40 ug, Lane 8: Esophagus (Rat) Lysate at 40 ug, Primary: Anti-Cytokeratin 5 (orb317705) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 62 kD, Observed band size: 60 kD.
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Protocol Information
Pan-cytokeratin Rabbit Polyclonal Antibody (orb317705)
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