PCNA Antibody (C-term)

SKU: orb34189

Description

Purified rabbit polyclonal antibody.

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Cancer,Neuroscience,Product Categories/Products/Antibodies/Primary Antibodies,Cancer/DNA Damage,Epigenetics,Infectious Diseases,Primary Antibodies;Cancer;Cell Biology

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution Range: WB: 1:1000, IHC-P: 1:10-50, IF/ICC: 1:10-50
• Reactivity: Human
• Predicted Reactivity: Hamster, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB17486
• Immunogen: Synthetic Peptide
• Target: PCNA
• Molecular Weight: 28769
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-Proliferating cell nuclear antigen antibody, anti-PCNA antibody, anti-Cyclin antibody

Western blot analysis of PCNA Antibody (C-term) in Jurkat, Hela, 293 cell line lysates (35 ug/lane). PCNA (arrow) was detected using the purified Pab.

Confocal immunofluorescent analysis of PCNA Antibody (C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Western blot analysis of PCNA (arrow) using rabbit polyclonal PCNA Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PCNA gene.

Confocal immunofluorescent analysis of PCNA Antibody (C-term) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PCNA antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

PCNA Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PCNA Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Quick Database Links

Gene Symbol

PCNA

UniProt

P12004

RefSeq (Protein):NP_002583.1, NP_872590.1