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Description
Research Area
,Recombinant-Protein
Images & Validation
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| Application Notes |
|---|
Key Properties
−| Expression System | HEK293 Cells |
|---|---|
| Biological Origin | Canine |
| Biological Activity | PD-1 Protein, Canine, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 43 kDa and the accession number is XP_543338.3. |
| Tag | C-hFc |
| Expression Region | A DNA sequence encoding the canine PDCD1 (XP_543338.3) (Met1-Leu169) was expressed with the Fc region of human IgG1 at the C-terminus. Predicted N terminal: Leu 25 |
| MW | 43 kDa (predicted) |
| Purity | 98.00% |
Storage & Handling
−| Storage | -20°C |
|---|---|
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
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Canine PD-1 Protein, hFc Tag [orb2321232]
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 42.3 kDa after removal of the signal peptide. The apparent molecular mass of dPD-1-hFc is approximately 55-70 kDa due to glycosylation.
Mammalian
100 μg, 10 μg, 50 μg PD-1 Protein, Canine, Recombinant (aa 25-170, hFc) [orb1960214]
98.00%
43.00 kDa (predicted). Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result.
500 μg, 100 μg, 1 mg
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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
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