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Description
Research Area
Images & Validation
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| Application Notes |
|---|
Key Properties
−| Expression System | HEK293 Cells |
|---|---|
| Biological Origin | Mouse |
| Biological Activity | PD-1 Protein, Mouse, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 42.9 kDa and the accession number is Q02242. |
| Tag | C-hFc |
| Expression Region | A DNA sequence encoding the mouse PDCD1 (NP_032824.1) (Met1-Gln167) was expressed with the Fc region of human IgG1 at the C-terminus. Predicted N terminal: Leu 25 |
| MW | 42.9 kDa (predicted) |
| Purity | SDS-PAGE: 96.4%; SEC-HPLC: 98.7% |
Storage & Handling
−| Storage | -20°C |
|---|---|
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
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−Mouse PD-1 Protein, hFc Tag [orb1826784]
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 42.3 kDa after removal of the signal peptide. The apparent molecular mass of mPD-1-hFc is approximately 35-70 kDa due to glycosylation.
Mammalian
10 μg, 50 μg, 100 μgPD-1 Protein (Primary Amine Labeling), Mouse, Recombinant (hFc), Biotinylated [orb1960699]
98.00%
42.9 kDa (predicted). Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
1 mg, 500 μg, 100 μg

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