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Description
Research Area
,Immunology,Cancer,Cell Cycle
Images & Validation
−Item 1 of 2
| Tested Applications | ELISA, FC |
|---|---|
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Recombinant |
| Isotype | sdAb |
| Clone No. | F2-5F4 |
| Immunogen | PD-1 antibody was raised against a recombinant protein corresponding to amino acids 25 - 167 of human PD-1. |
| Target | PDCD1 |
| Purification | PD-1 Antibody is affinity chromatography purified via Nickel column. Antibody is supplied as a His-tagged purified protein. It also contains a myc-tag for detection. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PD-1 Antibody is supplied in PBS. |
| Concentration | 1 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−PD-1 Antibody: PD1, PD-1, CD279, SLEB2, hPD-1, hPD-l, hSLE1, PD1, Programmed cell death protein 1, Protein PD-1, PDCD1, PDCD-1

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![PD-1 Single Domain Antibody [F25F4]](/images/pub/media/catalog/product/NewWebsite/15/orb1274202_1.gif)
Titration ELISA of PD-1.
![PD-1 Single Domain Antibody [F25F4]](/images/pub/media/catalog/product/NewWebsite/15/orb1274202_2.jpg)
Flow cytometry analysis of PD-1 transfected 293 cells using PD-1 [F2-5F4] antibody at 10 μg/ml. Blue: untransfected cells, Yellow: PD-1 transfected cells.
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Product Information
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Protocol Information
FC
Flow Cytometry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
PD-1 Single Domain Antibody [F25F4] (orb1274202)
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